Fluorescence Digital Pathology Scanner: Revolutionizing Diagnostic Detection | KFBIO

In the past, diagnosing diseases or pathogens often relied on immunochromatographic bands, which were limited by visual detection or basic absorbance measurements. Fluorescent immunoassay (FIA) has emerged as a superior in-vitro diagnostic (IVD) method, offering significantly higher sensitivity. The fluorescence digital pathology scanner is the cornerstone of this advanced detection technology.

A fluorescence digital pathology scanner is essential for high-precision fluorescence detection and rapid diagnostics. It uses specific excitation and emission wavelengths for various fluorophores to flexibly scan different samples. These scanners are renowned for their high sensitivity, high accuracy, ease of operation, and excellent cost-performance ratio.

They create high-resolution digital slide images using automated microscopes and professional cameras. Through high-precision fluorescence Whole Slide Imaging (WSI) stitching algorithms and professional image enhancement, they restore images with exceptional clarity. The data is stored in compressed electronic files, making it convenient for medical, research, and testing personnel to scale, read, measure, and annotate slides via software.

Common Challenges in Fluorescence Digital Scanning & The KFBIO Solution

KFBIO addresses the key limitations of standard fluorescence scanners with innovative engineering and certified clinical solutions.

Problem	KFBIO Solution
Lack of Clinical Certification: Most scanners are for research only, lacking Class II medical device certification.	Clinically Certified: KFBIO scanners hold the Class II medical device registration certificate, approved for clinical use, research, drug validation, FISH, and multiplex immunofluorescence.
Channel Crosstalk: Multi-label fluorescence scanning often suffers from color cross-talk and inaccurate color representation.	Precision Optical Filters: Uses CHROMA narrow-band filters for superior fluorescence separation. Ensures zero crosstalk, high brightness, and accurate color matching.
Poor Dark-Field Stitching: Weak WSI image stitching in dark fields of view, leading to poor image quality.	Advanced Stitching Algorithm: Employs a high-precision direct-position fluorescence WSI algorithm. Boasts a 50% higher control accuracy for seamless, high-quality image stitching.
Insufficient Resolution for FISH: Optical resolution is often inadequate for detecting small FISH and other probe signals.	Superior Optics & Imaging: Features top-tier dual objective lenses with a NA of 0.8 (20X) and NA of 0.95 (40X), among the highest in the industry. Combined with a low-noise sCMOS camera and enhancement algorithms for maximum resolution.
Unsuitable for Clinical Workflows: Difficulty automatically identifying tissue areas on transparent fluorescent slides.	Intelligent Area Detection: Uses original preview image capture technology to intelligently identify key tissue areas, ensuring fast, high-quality scans tailored for clinical practice.
Low Throughput & Manual Operation: Most scanners require constant monitoring and manual intervention, lacking true automation.	True Walk-Away Automation: Enables unattended, 24/7 scanning with a high success rate and stable performance for large-scale workloads.
Poor User Experience: Complex interfaces with poor human-computer interaction design.	User-Centric Design: Features an intuitive, customized operation interface optimized for clinical application scenarios.
Slow Scanning Speed: Imaging speed and throughput are insufficient for high-demand environments.	Industry-Leading Speed: Scans a slide in ≤7 minutes (40X, 3 channels) and supports batch scanning of up to 400 slides at a time with multiple channel options.

Key Advantages of KFBIO Fluorescence Scanners

KFBIO scanners are engineered with advanced hardware and intelligent software to outperform the market.

1. Customizable, High-Performance Filter Module

Supports up to 8 customizable channels for dense multi-color data without quality loss.

Equipped with CHROMA narrow-band filters with 10nm resolution, 99% transmittance, and OD6 cutoff.

2. Original LED Light Source Design

• Independent, specified-band LED sources prevent crosstalk.
• Delivers high contrast, high brightness, and zero drift for specific fluorescent channels.

3. Cutting-Edge sCMOS Camera

• Features the Dhyana400D with a high Quantum Efficiency of 80% at 600nm and extremely low readout noise (2e-).
• Large 26.5mm field of view and fast 35 fps speed enable high-sensitivity, low-light detection and rapid data transmission.

4. High-Precision Image Stitching & Optics

Advanced flat-field correction and image enhancement algorithms for superior image quality without manual slide correction.

Proprietary algorithms increase control accuracy by 50% for seamless WSI.

Top-tier objective lenses (NA 0.8 @20X, NA 0.95 @40X) near the air transmission limit.

5. Intelligent, Automated Scanning

• Original preview technology for automatic scan area identification, multiplex channel fusion, and multi-layer FISH analysis.
• Enables 24/7 unattended operation with automated quantitative analysis and reporting.

6. Flexible Hardware Configuration

• Supports combined fluorescence and bright-field scanning in one machine.
• Standard 3 or 6 channels, expandable to 10. Modules can be purchased independently for ultimate flexibility.
• Features removable filters for easy maintenance.

7. Enhanced Reliability & Long Service Life

• Long-life LED source (>20,000 hours).
• Incorporates non-contact Hall switches and wireless charging to reduce cables and wear, dramatically increasing system stability and lifespan.

8. Unmatched Scanning Speed & Throughput

• ≤7 minutes for a 10x10mm area at 40X with 3 channels.
• Capacity to handle up to 400 slides in a single batch.

Optimized for: Fluorescence Digital Scanner, FISH Scanning, Multiplex Immunofluorescence, Clinical Pathology, High-Resolution WSI, IVD Equipment, KFBIO Scanner.