Fluorescence Digital Pathology Scanner for FISH and Multiplex Immunofluorescence

High-Resolution Fluorescence Whole Slide Imaging for Clinical and Research Applications

Fluorescence imaging has become essential in modern pathology and biomedical research. It helps detect biomarkers, genetic abnormalities, and molecular targets with high sensitivity and accuracy. However, traditional immunochromatographic methods still rely on visual interpretation or simple absorbance measurements. As a result, they often provide limited sensitivity and quantitative information.

Fluorescent immunoassay (FIA) offers a more advanced solution. It provides higher sensitivity, stronger signal detection, and greater analytical accuracy. Therefore, many laboratories now use fluorescence imaging for both clinical diagnostics and research. A fluorescence digital pathology scanner plays a key role in this workflow. It converts fluorescent slides into high-resolution digital images for diagnosis, research, and quantitative analysis.

KFBIO fluorescence digital pathology scanners combine advanced optics, intelligent automation, and clinical-grade reliability. In addition, they support FISH, multiplex immunofluorescence (mIF), translational medicine, and complete digital pathology workflows.

What Is a Fluorescence Digital Pathology Scanner?

A fluorescence digital pathology scanner captures fluorescent signals from tissue samples and converts them into whole slide images (WSIs). It uses dedicated excitation and emission wavelengths for different fluorophores. As a result, the scanner accurately detects multiple biomarkers within a single specimen.

Compared with conventional fluorescence microscopy, a fluorescence whole slide scanner offers several important advantages. These include:

• Higher imaging efficiency
• More consistent image quality
• Automated slide processing
• Digital image storage
• Remote image sharing
• Quantitative image analysis

The scanner creates high-resolution digital slides through automated microscopy and advanced imaging technology. In addition, fluorescence whole slide imaging (WSI) algorithms create complete slide images with excellent clarity and accuracy.

Pathologists can easily view, measure, annotate, and share digital slides through pathology software platforms. As a result, laboratories improve collaboration, streamline workflows, and increase diagnostic efficiency.

Common Challenges in Fluorescence Digital Scanning & The KFBIO Solution

KFBIO addresses the key limitations of standard fluorescence scanners with innovative engineering and certified clinical solutions.

Problem	KFBIO Solution
Lack of Clinical Certification: Most scanners are for research only, lacking Class II medical device certification.	Clinically Certified: KFBIO scanners hold the Class II medical device registration certificate, approved for clinical use, research, drug validation, FISH, and multiplex immunofluorescence.
Channel Crosstalk: Multi-label fluorescence scanning often suffers from color cross-talk and inaccurate color representation.	Precision Optical Filters: Uses CHROMA narrow-band filters for superior fluorescence separation. Ensures zero crosstalk, high brightness, and accurate color matching.
Poor Dark-Field Stitching: Weak WSI image stitching in dark fields of view, leading to poor image quality.	Advanced Stitching Algorithm: Employs a high-precision direct-position fluorescence WSI algorithm. Boasts a 50% higher control accuracy for seamless, high-quality image stitching.
Insufficient Resolution for FISH: Optical resolution is often inadequate for detecting small FISH and other probe signals.	Superior Optics & Imaging: Features top-tier dual objective lenses with a NA of 0.8 (20X) and NA of 0.95 (40X), among the highest in the industry. Combined with a low-noise sCMOS camera and enhancement algorithms for maximum resolution.
Unsuitable for Clinical Workflows: Difficulty automatically identifying tissue areas on transparent fluorescent slides.	Intelligent Area Detection: Uses original preview image capture technology to intelligently identify key tissue areas, ensuring fast, high-quality scans tailored for clinical practice.
Low Throughput & Manual Operation: Most scanners require constant monitoring and manual intervention, lacking true automation.	True Walk-Away Automation: Enables unattended, 24/7 scanning with a high success rate and stable performance for large-scale workloads.
Poor User Experience: Complex interfaces with poor human-computer interaction design.	User-Centric Design: Features an intuitive, customized operation interface optimized for clinical application scenarios.
Slow Scanning Speed: Imaging speed and throughput are insufficient for high-demand environments.	Industry-Leading Speed: Scans a slide in ≤7 minutes (40X, 3 channels) and supports batch scanning of up to 400 slides at a time with multiple channel options.

How KFBIO Solves These Challenges

To overcome the limitations of conventional fluorescence imaging, KFBIO developed a clinical-grade fluorescence digital pathology scanner. It combines advanced optical technology, intelligent automation, and high-speed scanning to support both clinical and research applications.

Clinically Certified Solution

KFBIO fluorescence scanners hold Class II medical device certification. Therefore, they support clinical diagnostics, scientific research, drug development, FISH analysis, and multiplex immunofluorescence applications with confidence.

Precision Optical Filters

KFBIO uses premium CHROMA narrow-band optical filters to improve fluorescence imaging performance. These filters provide excellent fluorescence separation and accurate color reproduction.

As a result, users benefit from higher brightness, stronger signal integrity, and zero channel crosstalk.

Advanced Fluorescence WSI Stitching

KFBIO uses a proprietary high-precision fluorescence whole slide imaging (WSI) algorithm. It improves image control accuracy by 50%. Consequently, the scanner creates seamless whole slide images with excellent clarity and consistency.

Superior Imaging Performance

KFBIO scanners use industry-leading objective lenses to capture high-quality fluorescence images. The 20× objective offers a numerical aperture of 0.8. The 40× objective reaches a numerical aperture of 0.95.

In addition, the integrated low-noise sCMOS camera captures weak fluorescence signals with excellent clarity. This combination helps produce accurate and reliable imaging results.

Intelligent Tissue Detection

KFBIO developed an original preview imaging technology that automatically detects tissue regions before scanning. As a result, the scanner avoids unnecessary scanning areas, improves efficiency, and shortens overall scanning time.

Fully Automated Workflow

KFBIO scanners support unattended 24/7 operation. They automatically process large batches of slides with minimal user intervention. Therefore, laboratories can increase throughput while reducing routine manual work.

User-Friendly Operation

KFBIO designed the software interface for clinical pathology workflows. Users can quickly learn the system and begin routine operation with minimal training. Furthermore, the intuitive interface simplifies daily workflow management and improves overall productivity.

High-Speed Scanning

Speed is essential for high-volume laboratories. Therefore, KFBIO scanners complete one slide in as little as seven minutes at 40× using three fluorescence channels. In addition, the platform supports batch scanning of up to 400 slides. This helps laboratories handle large workloads while maintaining consistent image quality.

Key Advantages of KFBIO Fluorescence Digital Pathology Scanners

KFBIO fluorescence digital pathology scanners combine advanced imaging technology with intelligent automation. As a result, they deliver excellent image quality, high efficiency, and reliable performance for both clinical diagnostics and scientific research.

1. Customizable Multi-Channel Imaging

KFBIO supports up to eight customizable fluorescence channels. This flexibility allows researchers to perform complex multiplex immunofluorescence (mIF) studies without sacrificing image quality.

In addition, premium CHROMA optical filters provide outstanding optical performance, including:

• 10 nm spectral resolution
• 99% transmittance
• OD6 cutoff performance

Together, these features improve fluorescence separation and ensure accurate color reproduction.

2. Advanced LED Illumination System

KFBIO uses independent LED light sources for each fluorescence channel. As a result, the scanner eliminates channel interference and delivers stable illumination throughout every scan.

Users benefit from:

• High contrast
• High brightness
• Zero signal drift

Furthermore, the long-life LED design reduces maintenance requirements and improves long-term reliability.

3. High-Sensitivity sCMOS Camera

KFBIO integrates the Dhyana400D scientific sCMOS camera to capture weak fluorescence signals with excellent sensitivity.

Key specifications include:

• 80% quantum efficiency at 600 nm
• 2e⁻ ultra-low readout noise
• 26.5 mm field of view
• 35 fps acquisition speed

As a result, laboratories obtain clear fluorescence images even under low-light conditions.

4. High-Precision Imaging and Optics

KFBIO combines advanced flat-field correction with intelligent image enhancement algorithms. Together, these technologies improve image consistency and signal quality. In addition, proprietary fluorescence WSI stitching technology creates seamless whole slide images with high accuracy.

Industry-leading objective lenses further improve imaging performance:

• 20× objective with a numerical aperture of 0.8
• 40× objective with a numerical aperture of 0.95

This optical design captures more image details and delivers highly reliable fluorescence images.

5. Intelligent Automation

KFBIO automates the entire fluorescence scanning workflow. As a result, laboratories save time and reduce manual operation.

The platform supports:

• Automatic scan area detection
• Multiplex channel fusion
• Multi-layer FISH analysis
• Automated quantitative analysis
• Automated report generation

Therefore, users can achieve higher efficiency while maintaining consistent analysis quality.

6. Flexible Hardware Configuration

Every laboratory has different workflow requirements. Therefore, KFBIO offers flexible hardware configurations to meet different application needs.

Users can choose from:

• Standard 3-channel models
• Standard 6-channel models
• Expandable 10-channel systems

In addition, the scanner supports both brightfield and fluorescence imaging in one platform. Laboratories can also upgrade individual modules as their needs grow.

7. Long-Term Reliability

KFBIO designs its scanners for continuous operation in demanding laboratory environments. The LED light source provides more than 20,000 operating hours. In addition, non-contact Hall switches improve durability, while wireless charging technology reduces cable wear.

As a result, laboratories enjoy lower maintenance costs and greater long-term system stability.

8. Industry-Leading Throughput

High throughput is essential for busy pathology laboratories. KFBIO scanners complete a 10 × 10 mm scan in as little as seven minutes at 40× using three fluorescence channels. In addition, the platform processes up to 400 slides in a single batch.

Therefore, laboratories can increase productivity without compromising image quality or scanning accuracy.

Applications of Fluorescence Digital Pathology Scanners

KFBIO fluorescence scanners support a wide range of applications.

These include:

• Fluorescence in situ hybridization (FISH)
• Multiplex immunofluorescence (mIF)
• Cancer research
• Biomarker discovery
• Companion diagnostics
• Drug development
• Translational medicine
• Clinical pathology
• Precision medicine

The platform delivers reliable results for both research and clinical environments.

Why Choose KFBIO?

KFBIO is a global provider of digital pathology solutions. The company offers scanners, pathology software, image management platforms, and AI-assisted diagnostic tools.

Its fluorescence digital pathology scanners combine:

• Clinical-grade certification
• Advanced optical engineering
• Intelligent automation
• High-throughput performance
• Superior image quality

These capabilities help laboratories improve diagnostic efficiency and accelerate research outcomes.